Human PSMA-coupled Magnetic Beads

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MBP-K007-2mg
$1,315.00
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MBP-K007-10mg (5mg X 2)
$4,155.00
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MBP-K007
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Product Information
Items
Size 2mg
Size 10mg (5mg × 2)
Particle size
2 μm
2 μm
Physical appearance
Powder mixture
Powder mixture
Source
Lys 44 - Ala 750 (Accession # Q04609-1)
Lys 44 - Ala 750 (Accession # Q04609-1)
Amount of Coupled Protein
>277 pmol (20 μg) PSMA/mg beads
>277 pmol (20 μg) PSMA/mg beads
Formulation
PBS, pH7.4, with 10% Trehalose
PBS, pH7.4, with 10% Trehalose
Reconstitution
2 mL sterile deionized water (1 mg beads/mL)
5 mL sterile deionized water (1 mg beads/mL)
Product Description

The Human PSMA-coupled Magnetic Beads is produced by coupling biotinylated human PSMA protein to streptavidin-conjugated magnetic beads. The pre-coupled beads are ready to use for capturing anti-PSMA antibody from your sample with high specificity. The uniform size and large surface-to-volume ratio of the beads ensure highly efficent immunocapture in a simple, fast and convenient workflow with high reproducibilty of data.

Application

The beads is intended for immunocapture, biopanning. This is a non-sterile product.

* The Isotype control (Cat. No. SMB-B01) is sold separately.

Reconstitution

See Certificate of Analysis (CoA) for detailed instruction.

Storage

This product is stable in storage under the following conditions: -20℃ for 24 months in lyophilized state.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

Assay Principles

Antibody Purification:
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.