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Your Position: Home > Cytokines > IFN-alpha 1 > IFA-M52H3

Mouse IFN-alpha 1 Protein, His Tag

  • Synonym
    IFN-alpha 1,IFNA1,IFNalpha 1,IFN-alpha-1,LeIF D
  • Source
    Mouse IFN-alpha 1, His Tag (IFA-M52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Lys 189 (Accession # P01572-1).
    Predicted N-terminus: Cys 24
  • Molecular Characterization
    Online(Cys 24 - Lys 189) P01572-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.0 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse IFN-alpha 1, His Tag (Cat. No. IFA-M52H3) SDS-PAGE gel

Mouse IFN-alpha 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Interferon alpha-1 is also known as IFN-alpha-1/13, Interferon alpha-D, LeIF D and IFNA1, belongs to the alpha / beta interferon family. Interferons alpha-1 and alpha-13 have identical protein sequences. Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha can either suppress or promote the development of autoimmune diabetes. It is likely that IFN-alpha plays a complex role in the etiology of type 1 diabetes.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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