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Your Position: Home > Immune Checkpoint Proteins > CD229 > CD9-H82E6

Biotinylated Human CD229 / SLAMF3 Protein, His,Avitag™

  • Synonym
    SLAMF3,LY9,Ly-9,hly9,Ly9
  • Source
    Biotinylated Human CD229, His,Avitag (CD9-H82E6) is expressed from human 293 cells (HEK293). It contains AA Lys 48 - Lys 454 (Accession # Q9HBG7-1).
  • Molecular Characterization
    Online(Lys 48 - Lys  454) Q9HBG7-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 48.5 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD229, His,Avitag (Cat. No. CD9-H82E6) SDS-PAGE gel

Biotinylated Human CD229, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD229, His,AvitagBiotinylated Human CD229, His,Avitag (Cat. No. CD9-H82E6) ELISA bioactivity

Immobilized Human CD229, His Tag (Cat. No. CD9-H52H6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD229, His,Avitag (Cat. No. CD9-H82E6) with a linear range of 0.039-2.5 μg/mL (QC tested).

  • Background
    CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. Signaling lymphocyte activation molecule (SLAM) family receptors are critically involved in modulating innate and adaptive immune responses. CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells. Homophilic binding between CD229 molecules is mediated by the N-terminal Ig-like domain.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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