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Product Details
- ProductSizeAmountActiveMax® Human CD229 μBeads, premium grade (for cells)2.5 mg2.5×10⁷ beads
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This product can be directly isolated using conventional column-free magnetic stand (eg. Cat. MB-01/ MB-02/ MB-03/ MB-04). Please note that due to its micron-scale size, it is not compatible with commercially available column-based (M/D/L) magnetic separation systems.
Product Description
ActiveMax® Human CD229 μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm in diameter immobilized with Human CD229 / SLAMF3 protein expressed from human 293 cells (HEK293) and contains AA Lys 48 - Lys 454 (Accession # Q9HBG7-1).
ActiveMax® Human CD229 μBeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.Application
ActiveMax® Human CD229 μBeads, premium grade (for cells) are designed to stimulate in vitro CD229-specific CAR-T cells or UCAR-T cells, similar to the tumor cell lines that express human CD229 antigen. It can be used as follows:
Evaluating the characteristics of CD229-specific CAR-T cells or UCAR-T cells.
In vitro expansion of CD229-specific CAR-T cells or UCAR-T cells.
In vitro enrichment of CD229-specific CAR-T cells or UCAR-T cells.Reconstitution
See Certificate of Analysis (CoA) for detailed instruction.
Storage
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please protect from light and avoid repeated freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Sterility
Negative
Endotoxin
Less than 0.002 EU per μg by the LAL method / rFC method.
Mycoplasma
Negative
Important Note
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
Formulation
Lyophilized in PBS with 0.1% HSA，pH 7.4. Trehalose is added as protectant before lyophilization.
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Performance Data
FACS Data
Assay of human CD229 protein on the μBeads surface by Flow cytometry. The human CD229 protein conjugated on the μBeads (Cat. No. MBS-C010) surface were fluorescently stained using PE anti-human CD229 Antibody and analyzed by flow cytometry.
Protocol
Stimulate CD229 CAR-T Cells -Cytokines
CD229 CAR-T cells were stimulated with ActiveMax® Human CD229 μBeads (Cat. No. MBS-C010) for 24 h , and cell-free supernatants were harvested for evaluating IFN-γ、TNF-α、IL-4、IL-6 secretion by flow cytomtry. The results showed that CD229 CAR-T cells released significantly larger amounts of IFN-γ、TNF-α、IL-4、IL-6 into the supernatants in response to CD229 μBeads (Data is the average of 3 independent experiments, error bars ± SD).
Stimulate CD229 CAR-T Cells -Cytotoxic Molecules
CD229 CAR-T cells were stimulated with ActiveMax® Human CD229 μBeads (Cat. No. MBS-C010) for 24h, and cell-free supernatants were harvested for evaluating Granzyme B secretion by ELISA. The results showed that CD229 CAR-T cells released significantly larger amounts of Granzyme B into the supernatants in response to CD229 μBeads (Data is the average of 3 independent experiments, error bars ± SD).
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