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Your Position: Home > All Other Proteins > Apo-A1 > Human Apo-A1 Protein, His Tag

Human Apo-A1 Protein, His Tag

  • Synonym
    Apolipoprotein A-I,APOA1,MGC117399
  • Source
    Human Apo-A1, His Tag (AP1-H5225) is expressed from human 293 cells (HEK293). It contains AA Arg 19 - Gln 267 (Accession # NP_000030.1).
    Predicted N-terminus: Arg 19
  • Molecular Characterization
    Online(Arg 19 - Gln 267) NP_000030.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 29.8 kDa. The protein migrates as 26-30 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Apo-A1, His Tag (Cat. No. AP1-H5225) SDS-PAGE gel

Human Apo-A1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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