This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 139.7 kDa. The protein migrates as 180-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated SARS-CoV-2 S protein, His,Avitag, Super stable trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated SARS-CoV-2 S protein, His Tag, Super stable trimer(Cat. No. SPN-C82E9) is more than 90% and the molecular weight of this protein is around 520-620 kDa verified by SEC-MALS.
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein, His,Avitag, Super stable trimer (Cat. No. SPN-C82E9) with a linear range of 0.004-0.125 μg/mL (QC tested).
Immobilized Biotinylated SARS-CoV-2 S protein, His,Avitag, Super stable trimer (Cat. No. SPN-C82E9) at 1 μg/mL (100 μL/well)on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate. can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-2 ng/mL (Routinely tested).
Biotinylated SARS-CoV-2 S protein, His,Avitag, Super stable trimer (Cat. No. SPN-C82E9) captured on Biotin CAP - Series S sensor Chip can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 20.1 nM as determined in a SPR assay (Biacore T200).
Authors: Troyer, Zach et al.
Journal: Journal of extracellular vesicles vol 2021
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