This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 20.9 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human 4-1BB, His,Avitag™(MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Human 4-1BB, His,Avitag™(MALS verified) (Cat. No. 41B-H82E6) was more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Immobilized Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. 41L-H52D4) at 1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, His,Avitag™(MALS verified) (Cat. No. 41B-H82E6) with a linear range of 1-39 ng/mL (QC tested).
Immobilized Biotinylated Human 4-1BB, His,Avitag™(MALS verified) (Cat. No. 41B-H82E6) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate, can bind Human 4-1BB Ligand, Fc Tag (Cat. No. 41L-H5257) with a linear range of 0.1-2 ng/mL (Routinely tested).
Biotinylated Human 4-1BB Protein, His,Avitag™(MALS verified) (Cat. No. 41B-H82E6) captured on Biotin CAP-Series S sensor Chip can bind anti 4-1BB mAb with an affinity constant of 0.158 nM as determined in a SPR assay (Biacore T200) (Routinely tested) .
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $1380.00
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