This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 52.5 kDa. The protein migrates as 55-60 kDa under non-reducing (NR) condition (SDS-PAGE) due to Glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH 6.0. Normally Trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human BAFF, His,Flag Tag, active trimer on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) was more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.
Immobilized Human BAFFR, Fc Tag (Cat. No. BAR-H5257) at 2 μg/mL (100 μL/well) can bind Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) with a linear range of 4-63 ng/mL when detected by Monoclonal anti FLAG (HRP) antibody (QC tested).
Immobilized Human TACI/TNFRSF13B, Fc Tag (Cat. No. TAI-H5256) at 2 μg/mL (100 μL/well) can bind BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) with a linear range of 4-31 ng/mL when detected by Monoclonal anti FLAG (HRP) antibody(Routinely tested).
Human BAFFR Protein, Fc Tag (Cat. No. BAR-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFF Protein, His,Flag Tag (Cat. No. BAF-H52D4) with an affinity constant of 5.54 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) stimulates proliferation of mouse spleen cells. The EC50 for this effect is 1.97-2.90 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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