>95% as determined by SDS-PAGE.
Delivered as bulk protein in a 0.2 μm filtered solution of PBS, pH7.4.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored in liquid state at 2-6°C upon receipt.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H2) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) with a linear range of 0.08-2.5 ng/mL (QC tested).
Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike S1 Antibody, chimeric mAb (Cat. No. S1N-M12A1) at 2 μg/mL (100 μL/well) can bind S protein RBD. And then add Biotinylated Anti-SARS-CoV-2 Spike S1 Antibody (Cat. No. S1N-M13A1) at 1:5000. Detection was performed using HRP-conjugated streptavidin with sensitivity of 25 pg/mL (Routinely tested).
Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody, Mouse IgG1 (Cat. No. S1N-M12A1) and 0.4 μg/mL (100 μL/well) Biotinylated S protein RBD, His,Avitag (Cat. No. SPD-C82E9) with a half maximal inhibitory concentration (IC50) of 0.32 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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