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Your Position: Home > All Other Proteins > Transferrin R > TFR-H82E5

Biotinylated Human Transferrin R / CD71 Protein, His,Avitag™ (MALS verified)

  • Synonym
    CD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90
  • Source
    Biotinylated Human Transferrin R, His,Avitag (TFR-H82E5) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # P02786-1).
    Predicted N-terminus: Gly
  • Molecular Characterization
    Online(Cys 89 - Phe 760) P02786-1

    This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 78.7 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Transferrin R, His,Avitag (Cat. No. TFR-H82E5) SDS-PAGE gel

Biotinylated Human Transferrin R, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human Transferrin R, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human Transferrin R, His,Avitag(Cat. No. TFR-H82E5) was more than 90% and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human Transferrin R, His,AvitagBiotinylated Human Transferrin R, His,Avitag (Cat. No. TFR-H82E5) ELISA bioactivity

Immobilized Human Transferrin, His Tag (Cat. No. TRN-H4229) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R, His,Avitag (Cat. No. TFR-H82E5) with a linear range of 2-63 ng/mL (QC tested).

  • Background
    CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 2 Clinical

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