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Your Position: Home > Viral Proteins > S protein RBD > SPD-C82Aa

Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified)

  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD,COVID-19
  • Source
    Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (SPD-C82Aa) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1).
    Predicted N-terminus: Arg 319
  • Molecular Characterization
    Online(Arg 319 - Lys 537) QHD43416.1

    This protein carries a mouse IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 52.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (Cat. No. SPD-C82Aa) SDS-PAGE gel

Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (Cat. No. ) MALS images

The purity of Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (Cat. No. SPD-C82Aa) was more than 90% and the molecular weight of this protein is around 105-120 kDa kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified)Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (Cat. No. SPD-C82Aa) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (Cat. No. SPD-C82Aa) with a linear range of 0.2-10 ng/mL (QC tested).

Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified)Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (Cat. No. SPD-C82Aa) ELISA bioactivity

Immobilized Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (Cat. No. SPD-C82Aa) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5μg/well) plate, can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) ) with a linear range of 0.2-3 ng/mL (Routinely tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $380.00

    Price(USD) : $1570.00

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    Datasheet & Documentation

    Request for DMF
    DMF (Drug Master File)

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