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Product Details
Synonyms
SDC1, Syndecan-1, CD138, SYND1, SDC
Source
Biotinylated Human Syndecan-1, His,Avitag (SY1-H82E2) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).
Predicted N-terminus: Gln 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 27.5 kDa. The protein migrates as 44-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human Syndecan-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate, can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) with a linear range of 2-63 ng/mL (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Syndecan-1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 1 Clinical
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