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Your Position: Home > All Other Proteins > Syndecan-1 > SY1-H82E2

Biotinylated Human Syndecan-1 Protein, His,Avitag™

  • Synonym
    SDC1,Syndecan-1,CD138,SYND1,SDC
  • Source
    Biotinylated Human Syndecan-1, His,Avitag™ (SY1-H82E2) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).
    Predicted N-terminus: Gln 23
  • Molecular Characterization
    Online( Gln 23 - Gly 254) P18827-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 27.5 kDa. The protein migrates as 43-55 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Syndecan-1, His,Avitag™ (Cat. No. SY1-H82E2) SDS-PAGE gel

Biotinylated Human Syndecan-1, His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human Syndecan-1, His,Avitag™Biotinylated Human Syndecan-1, His,Avitag™ (Cat. No. SY1-H82E2) ELISA bioactivity

Immobilized Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate, can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.4-6 ng/mL (QC tested).

Biotinylated Human Syndecan-1, His,Avitag™Biotinylated Human Syndecan-1, His,Avitag™ (Cat. No. SY1-H82E2) ELISA bioactivity

Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well)onstreptavidin (Cat. No. STN-N5116) (0.1 μg/well) plate, can bind Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) with a linear range of 2-63 ng/mL (Routinely tested).

  • Background
    Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $1380.00

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