This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 27.5 kDa. The protein migrates as 44-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Syndecan-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate, can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.4-6 ng/mL (QC tested).
2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human Syndecan-1, His,Avitag (Cat. No. SY1-H82E2) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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