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Your Position: Home > CAR-T > Syndecan-1 > SY1-H82F3

Biotinylated Human Syndecan-1 Protein, Fc,Avitag™

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    SDC1,Syndecan-1,CD138,SYND1,SDC
  • Source
    Biotinylated Human Syndecan-1, Fc,Avitag (SY1-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).
    Predicted N-terminus: Gln 23
  • Molecular Characterization
    Online( Gln 23 - Gly 254) P18827-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 52.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) SDS-PAGE gel

Biotinylated Human Syndecan-1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human Syndecan-1, Fc,AvitagBiotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) ELISA bioactivity

Immobilized Biotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate. can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.2-6 ng/mL (QC tested).

Biotinylated Human Syndecan-1, Fc,AvitagBiotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) ELISA bioactivity

Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) with a linear range of 0.5-8 ng/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 0.3 μg/mL of Biotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • Background
    Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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