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Your Position: Home > All Other Proteins > CD3 epsilon > CDE-C5226

Cynomolgus CD3 epsilon Protein, His Tag (MALS verified)

  • Synonym
    FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
  • Source
    Cynomolgus CD3 epsilon, His Tag (CDE-C5226) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    Online(Gln 22 - Asp 117) Q95LI5-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.1 kDa. As a result of glycosylation, the protein migrates as 18-23 kDa under reducing (R) condition, and 33-40 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) SDS-PAGE gel

Cynomolgus CD3 epsilon, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Cynomolgus CD3 epsilon, His Tag (Cat. No. ) MALS images

The purity of Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) was more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus CD3 epsilon, His TagCynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) ELISA bioactivity

Immobilize Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA), and then add Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection is performed using HRP-conjugated streptavidin. Binding occurs at a linear range of 1-5 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Anti-Human CD3 mAb, mouse IgG1 (Clone # SP34-2) on AMC Biosensor, can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.305 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Bispecific T-cell Engager (CD3 X BCMA) on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.113 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2100.00

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