This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.1 kDa. As a result of glycosylation, the protein migrates as 18-23 kDa under reducing (R) condition, and 33-40 kDa under non-reducing (NR) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus CD3 epsilon, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) was more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
Immobilize Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA), and then add Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection is performed using HRP-conjugated streptavidin. Binding occurs at a linear range of 1-5 ng/mL (QC tested).
Loaded Anti-Human CD3 mAb, mouse IgG1 (Clone # SP34-2) on AMC Biosensor, can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.305 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Bispecific T-cell Engager (CD3 X BCMA) on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.113 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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