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Your Position: Home > All Other Proteins > Syndecan-1 > SD1-HF228

FITC-Labeled Human Syndecan-1 Protein, His Tag

  • Synonym
    SDC1,Syndecan-1,CD138,SYND1,SDC
  • Source
    FITC-Labeled Human Syndecan-1, His Tag (SD1-HF228) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).
    Predicted N-terminus: Gln 23
  • Molecular Characterization
    Online( Gln 23 - Gly 254) P18827-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.8 kDa. The protein migrates as 42-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1.5-3.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human Syndecan-1, His Tag (Cat. No. SD1-HF228) SDS-PAGE gel

FITC-Labeled Human Syndecan-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
FITC-Labeled Human Syndecan-1, His TagFITC-Labeled Human Syndecan-1, His Tag (Cat. No. SD1-HF228) ELISA bioactivity

Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Syndecan-1, His Tag (Cat. No. SD1-HF228) with a linear range of 0.078-2.5 μg/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human Syndecan-1, His Tag (Cat. No. SD1-HF228) and negative control protein respectively, FITC signals was used to evaluate the binding activity (QC tested).

  • Background
    Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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