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Your Position: Home > All Other Proteins > CLEC12A > CLA-H82E6

Biotinylated Human CLEC12A / MICL / CLL-1 Protein, His,Avitag™

  • Synonym
    CLEC12A,MICL,CLL-1,CLL1,DCAL2,DCAL-2,CD371
  • Source
    Biotinylated Human CLEC12A, His,Avitag (CLA-H82E6) is expressed from human 293 cells (HEK293). It contains AA His 65 - Ala 265 (Accession # Q5QGZ9-2).
    Predicted N-terminus: Gly
  • Molecular Characterization
    Online(His 65 - Ala 265) Q5QGZ9-2

    This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 27.3 kDa. The protein migrates as 35-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CLEC12A, His,Avitag (Cat. No. CLA-H82E6) SDS-PAGE gel

Biotinylated Human CLEC12A, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human CLEC12A, His,AvitagBiotinylated Human CLEC12A, His,Avitag (Cat. No. CLA-H82E6) ELISA bioactivity

Immobilized Biotinylated Human CLEC12A, His,Avitag (Cat. No. CLA-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CLEC12A Antibody, Human IgG1 with a linear range of 0.1-0.8 ng/mL (QC tested).

Biotinylated Human CLEC12A, His,AvitagBiotinylated Human CLEC12A, His,Avitag (Cat. No. CLA-H82E6) ELISA bioactivity

Immobilized Monoclonal Anti-Human CLEC12A Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CLEC12A, His,Avitag (Cat. No. CLA-H82E6) with a linear range of 5-78 ng/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-CLEC12A CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Human CLEC12A, His,Avitag (Cat. No. CLA-H82E6) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • Background
    CLEC12A (C-type lectin domain family 12 member A) is also known as CLL1, DCAL2, MICL. Clec12a is an inhibitory receptor for uric acid crystals that regulates inflammation in response to cell death. Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. Evidence of distinct disease propagating stem cells in myelodysplastic syndrome (MDS) has emerged in recent years. The role of CLEC12A in MDS, however, remains to be elucidated. Furthermore, CLEC12A has been proposed as a promising marker of leukaemic stem cells in AML.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:5 Details
  • Latest Research Phase:Phase 3 Clinical

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