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Your Position: Home > Immune Checkpoint Proteins > CD27 Ligand > CDD-HF2D4

FITC-Labeled Human CD27 Ligand / CD70 Protein, His,Flag Tag active trimer (MALS verified)

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    FITC-Labeled Human CD27 Ligand, His,Flag Tag (CDD-HF2D4) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1). It is the biotinylated form of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No.CDL-H52Da).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Ser 52 - Pro 193) P32970-1

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

    The protein has a calculated MW of 49.3 kDa. The protein migrates as 50-55 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 2-4.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human CD27 Ligand, His,Flag Tag (Cat. No. CDD-HF2D4) SDS-PAGE gel

FITC-Labeled Human CD27 Ligand, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
FITC-Labeled Human CD27 Ligand, His,Flag Tag (Cat. No. ) MALS images

The purity of FITC-Labeled Human CD27 Ligand, His,Flag Tag (Cat. No. CDD-HF2D4) was more than 85% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.

Bioactivity-ELISA
FITC-Labeled Human CD27 Ligand, His,Flag TagFITC-Labeled Human CD27 Ligand, His,Flag Tag (Cat. No. CDD-HF2D4) ELISA bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Anti-CD27 Ligand Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD27 Ligand, His,Flag Tag (Cat. No. CDD-HF2D4) with a linear range of 0.039-0.156 μg/mL (QC tested).

FITC-Labeled Human CD27 Ligand, His,Flag TagFITC-Labeled Human CD27 Ligand, His,Flag Tag (Cat. No. CDD-HF2D4) ELISA bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human CD27, Fc Tag (Cat. No. CD7-H5254) at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human CD27 Ligand, His,Flag Tag (Cat. No. CDD-HF2D4) with a linear range of 0.039-0.156 μg/mL (Routinely tested).

  • Background
    Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $319.00

    Price(USD) : $1980.00

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