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Product Details
Synonyms
CTLA4, CD152
Source
Human CTLA-4, His Tag (CT4-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # P16410-1).
Predicted N-terminus: Ala 37
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.4 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 6 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human CTLA-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human CTLA-4, His Tag (Cat. No. CT4-H5229) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 1-6.4 ng/mL (Routinely tested).
Protocol
Immobilized Cadonilimab at 1 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Human CTLA-4, His Tag (Cat. No. CT4-H5229) at 5 μg/mL, add increasing concentrations of Cadonilimab (PD-1 x CTLA-4 bispecific antibody) and then add Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) at 0.2 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) with sensitivity of 3.07 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with an affinity constant of 25.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
J***o2023-02-03
LikesBought as potential coating protein to be used in ELISA to detected expressed Anti-CTLA-4. Vials arrived promptly and very well packaged. The website is informative and very intuitive to navigate to be able to find the product best suited for your needs. MSDS, CofA and Dataheets are readily available and contain accurate and comprehensive information e.g. storage temperatures, glycosylation information and purity- the guidance for usage of the protein was especially useful to serve as a jumping off point for coating concentrations. Results so far have been very promising which is what I have come to expect from ACROBiosystems- great work!- J***r2022-01-11 LikesThe product came in packaged properly and with proper documentation. CoAs were clear as to when the product would expire, both in the lyophilized state and reconstituted. Instructions for reconstitution were also clear. The packaging made it easy to quickly transfer the product to the appropriate storage. Package was shipped in a reasonable timeline. Details for the product were easy to find, which was extremely helpful for entering into our system.
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
Cytotoxic T-Lymphocyte-Associated Antigen 4
- Category：
- Approved Drugs：
5 Details
- Drugs in Clinical Trials：
65 Details
- Highest Development Stage：
Approved
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