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Your Position: Home > Viral Proteins > S protein > SPN-C82E3

Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)

  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein,COVID-19
  • Source
    Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (SPN-C82E3) is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1(D614G)) and D614G mutation, which has become increasingly common in SARS-CoV-2 viruses from around the world .
    The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus.
    Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
    It is the biotinlynated form of SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified) (Cat. No. SPN-C52H3).
    Predicted N-terminus: Val 16
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 139.7 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (Cat. No. SPN-C82E3) SDS-PAGE gel

Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (Cat. No. ) MALS images

The purity of Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (Cat. No. CPN-C82E3) was more than 90% and the molecular weight of this protein is around 520-620 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimerBiotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (Cat. No. SPN-C82E3) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (Cat. No. SPN-C82E3) with a linear range of 2-63 ng/mL (QC tested).

Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimerBiotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (Cat. No. SPN-C82E3) ELISA bioactivity

Immobilized Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer (Cat. No. SPN-C82E3) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-2 ng/mL (Routinely tested).

  • Background
    It's been reported that Coronavirus  can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $380.00

    Price(USD) : $1570.00

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