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Your Position: Home > All Other Proteins > NOTCH2 > NO2-H5255

Human NOTCH2 Protein, Fc Tag (MALS verified)

  • Synonym
    NOTCH2,hN2,N2ECD
  • Source
    Human NOTCH2, Fc Tag (NO2-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Gln 530 (Accession # Q04721-1).
    Predicted N-terminus: Leu 26
  • Molecular Characterization
    Online(Leu 26 - Gln 530) Q04721-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 80.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) SDS-PAGE gel

Human NOTCH2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human NOTCH2, Fc Tag (Cat. No. ) MALS images

The purity of Human NOTCH2, Fc Tag(Cat. No. NO2-H5255) was more than 85% and the molecular weight of this protein is around 175-190 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human NOTCH2, Fc TagHuman NOTCH2, Fc Tag (Cat. No. NO2-H5255) ELISA bioactivity

Immobilized Human DLL4, His Tag (Cat. No. DL4-H5227) at 5 μg/mL (100 μL/well) can bind Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) with a linear range of 5-78 ng/mL (QC tested).

Human NOTCH2, Fc TagHuman NOTCH2, Fc Tag (Cat. No. NO2-H5255) ELISA bioactivity

Immobilized Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (NOTCH2 × NOTCH3) with a linear range of 1-20 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) on Protein A Biosensor, can bind Human DLL4, His Tag (Cat. No. DL4-H5227) with an affinity constant of 77.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus . Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation . Positively regulates self-renewal of liver cancer cells .
  • References
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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