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Product Details
Synonyms
NOTCH2, hN2, N2ECD
Source
Human NOTCH2, Fc Tag (NO2-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Gln 530 (Accession # Q04721-1).
Predicted N-terminus: Leu 26
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Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 80.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human NOTCH2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) is more than 85% and the molecular weight of this protein is around 175-190 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DLL4, His Tag (Cat. No. DL4-H5227) at 5 μg/mL (100 μL/well) can bind Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Immobilized Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (NOTCH2 × NOTCH3) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human NOTCH2, Fc Tag (Cat. No. NO2-H5255) on Protein A Biosensor, can bind Human DLL4, His Tag (Cat. No. DL4-H5227) with an affinity constant of 77.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Neurogenic locus notch homolog protein 2
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Preclinical
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