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Your Position: Home > All Other Proteins > NOTCH3 > NO3-H5255

Human NOTCH3 Protein, Fc Tag

  • Synonym
    Notch-3,NOTCH3
  • Source
    Human NOTCH3, Fc Tag (NO3-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 40 - Glu 467 (Accession # Q9UM47-1).
  • Molecular Characterization
    Online(Ala 40 - Glu 467) Q9UM47-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 71.4 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human NOTCH3, Fc Tag (Cat. No. NO3-H5255) SDS-PAGE gel

Human NOTCH3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human NOTCH3, Fc TagHuman NOTCH3, Fc Tag (Cat. No. NO3-H5255) ELISA bioactivity

Immobilized Human NOTCH3, Fc Tag (Cat. No. NO3-H5255) at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (NOTCH2 × NOTCH3) with a linear range of 2-20 ng/mL (QC tested).

  • Background
    Notch3 is one of four mammalian Notch proteins, which act as signalling receptors and involve in developmental patterning, cell fate decisions, regulation of cell survival and proliferation. Notch3 could interact with membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Via ligand activation, notch intracellular domain (NICD) is released and forms a transcriptional activator RBPJ/RBPSUH complex, which activates genes of the enhancer of split locus. Consequently, the activation of Notch3 can lead to the implementation of differentiation, proliferation and apoptotic programs
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 2 Clinical

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