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Your Position: Home > Immune Checkpoint Proteins > MSP1D1 > APO-H81Q5

Biotinylated Human MSP1D1 Protein, His,Avitag™ (Nanodisc)

  • Synonym
    MSP1D1
  • Source
    Biotinylated Human MSP1D1, His,Avitag™ (Nanodisc) is expressed from E.coli cells. It contains AA Ser 1- Gln 211.
    Predicted N-terminus: Met
  • Molecular Characterization

    Biotinylated Human MSP1D1, His,Avitag™ (Nanodisc) (APO-H81Q5) is an empty nanodisc and assembles from a mixture of phospholipid micelles and membrane scaffold protein(MSP1D1).
    This protein carries a polyhistidine tag at the N-terminusa, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 29.4 kDa. The protein migrates as 28 kDa under reducing (R) condition (SDS-PAGE).
    This protein is used together with Biotinylated CD20 nanodisc protein (CD0-H82E3) as isotype control.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human MSP1D1, His,Avitag™ (Nanodisc) (Cat. No. APO-H81Q5) SDS-PAGE gel

Biotinylated Human MSP1D1, His,Avitag™ (Nanodisc) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 10 μg/mL of Biotinylated Human MSP1D1 Protein, His,Avitag™ (Nanodisc) (Cat. No. APO-H81Q5), washed and then followed by PE-SA and analyzed with FACS. PE-SA was used as negative control (QC tested).

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Price(USD) : $120.00

Price(USD) : $360.00

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