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Your Position: Home > Protein > MSP1D1 > APO-H81Q5

Biotinylated Human MSP1D1 Protein, His,Avitag™ (Nanodisc)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

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  • Synonym
    MSP1D1
  • Source
    Biotinylated Human MSP1D1 Protein, His,Avitag is expressed from E. coli cells. It contains AA Ser 1- Gln 211.
    Predicted N-terminus: Met
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminusa, followed by an Avi tag (Avitag™). The protein has a calculated MW of 29.4 kDa. The protein migrates as 28 kDa under reducing (R) condition (SDS-PAGE).

    This protein is used together with nanodisc protein as isotype control.

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
MSP1D1 SDS-PAGE

Biotinylated Human MSP1D1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-FACS
 MSP1D1 FACS

2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 10 μg/mL of Biotinylated Human MSP1D1 Protein, His,Avitag (Cat. No. APO-H81Q5), washed and then followed by PE-SA and analyzed with FACS. PE-SA was used as negative control (QC tested).

  • Background
    Membrane scaffold proteins (MSPs) are synthetic derivatives of apolipoprotein A-I, a major protein component of human high-density lipoprotein complexes. Membrane scaffold protein 1D1 (MSP1D1) is the most common one among the MSPs variants. MSP1D1 is a synthetic derivate of apolipoprotein A-I, which is the major protein element of human high-density lipoproteins. The amphipathic, synthetic protein has the ability to self-assemble in the presence of synthetic phospholipids into discoidal nanoparticles, so called nanodiscs.
  • Clinical and Translational Updates

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