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Your Position: Home > Membrane Protein > MSP1D1 > APO-H81Q5

Biotinylated Human MSP1D1 Protein, His,Avitag™ (Nanodisc)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    MSP1D1
  • Source
    Biotinylated Human MSP1D1, His,Avitag™ (Nanodisc) is expressed from E.coli cells. It contains AA Ser 1- Gln 211.
    Predicted N-terminus: Met
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminusa, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 29.4 kDa. The protein migrates as 28 kDa under reducing (R) condition (SDS-PAGE).
    This protein is used together with nanodisc protein as isotype control.

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human MSP1D1, His,Avitag™ (Nanodisc) (Cat. No. APO-H81Q5) SDS-PAGE gel

Biotinylated Human MSP1D1, His,Avitag™ (Nanodisc) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 10 μg/mL of Biotinylated Human MSP1D1 Protein, His,Avitag™ (Nanodisc) (Cat. No. APO-H81Q5), washed and then followed by PE-SA and analyzed with FACS. PE-SA was used as negative control (QC tested).

  • Background
    Membrane scaffold proteins (MSPs) are synthetic derivatives of apolipoprotein A-I, a major protein component of human high-density lipoprotein complexes. Membrane scaffold protein 1D1 (MSP1D1) is the most common one among the MSPs variants. MSP1D1 is a synthetic derivate of apolipoprotein A-I, which is the major protein element of human high-density lipoproteins. The amphipathic, synthetic protein has the ability to self-assemble in the presence of synthetic phospholipids into discoidal nanoparticles, so called nanodiscs.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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