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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-C52W9

Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDD-C52W9) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5-1(CD3E) & Q95LI8-1(CD3D)).
    Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D)) Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)

    Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.5 kDa (CD3E) and 40.9 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 46-50 kDa and 55-65 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-C52W9) SDS-PAGE gel

Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. ) MALS images

The purity of Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No.CDD-C52W9) was more than 90% and the molecular weight of this protein is around 105-120 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag TagCynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-C52W9) ELISA bioactivity

Immobilized Bispecific CD3×BCMA T cell-engaging Antibody at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-C52W9) with a linear range of 1-20 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-C52W9) on AHC Biosensor, can bind Bispecific CD3×BCMA T cell-engaging Antibody with an affinity constant of 1.77 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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