Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.5 kDa (CD3E) and 40.9 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 46-50 kDa and 55-65 kDa due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No.CDD-C52W9) was more than 90% and the molecular weight of this protein is around 105-120 kDa verified by SEC-MALS.
Immobilized Bispecific CD3×BCMA T cell-engaging Antibody at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-C52W9) with a linear range of 1-20 ng/mL (QC tested).
Loaded Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-C52W9) on AHC Biosensor, can bind Bispecific CD3×BCMA T cell-engaging Antibody with an affinity constant of 1.77 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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