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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-H82F6

Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His,Avitag™&Fc,Flag,Avitag™ (MALS verified)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta , epsilon
  • Source
    MABSol® Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (CDD-H82F6) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1(CD3E) & P04234-1(CD3D)). It is the biotinylated form of Human CD3 epsilon & CD3 delta Protein.
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) P07766-1 (CD3E) & P04234-1 (CD3D)

    Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag is produced by co-expression of CD3E and CD3D, has a calculated MW of 45.1kDa (CD3E) & 42.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™), the polyhistidine tag is inserted in-between the Fc and Avi tags at the C-terminus and subunit CD3D contains a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™), the flag tag is inserted in-between the Fc and Avi tags at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 50-60 kDa under reducing (R) condition.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) SDS-PAGE gel

Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No.CDD-H82F6) was more than 90% and the molecular weight of this protein is around 95-110 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,AvitagBiotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) ELISA bioactivity

Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.4-3 ng/mL (QC tested).

Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,AvitagBiotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) ELISA bioactivity

Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind UCHT1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) on SA Biosensor, can bind Bispecific CD3×BCMA T cell-engaging Antibody with an affinity constant of 1.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) on SA Biosensor, can bind UCHT1 with an affinity constant of 21.3 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag (Cat. No. CDD-H82F6) on SA Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with an affinity constant of 1.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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