This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 71.3 kDa. The protein migrates as 80-100 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Her2 (S310Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Her2 (S310Y), His Tag(Cat. No. HE2-H52H3) is more than 85% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.
Immobilized Human Her2 (S310Y), His Tag (Cat. No. HE2-H52H3) at 1 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.1-3 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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