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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.8 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Syndecan-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Syndecan-1, His Tag (Cat. No. SY1-H5225) at 1 μg/mL (100 μL/well) can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human Syndecan-1, His Tag (Cat. No. SY1-H5225) with a linear range of 2-125 ng/mL (Routinely tested).
2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human Syndecan-1, His Tag (Cat. No. SY1-H5225) and negative control protein respectively, washed and then followed by PE anti-His Tag antibody and analyzed with FACS (Routinely tested).
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