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Product Details
Synonyms
RPL, Protein L
Source
Biotinylated Recombinant Protein L, His,Avitag, also known as Recombinant Peptostreptococcus magnus Protein L, was expressed in E. coli at ACRObiosystems.
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 43.7 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE).
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Recombinant Protein L, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) is more than 95% and the molecular weight of this protein is around 40-56 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse Human chimeric Anti-BCMA mAb (Aa01, Mouse IgG1) at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Ipilimumab at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Immobilized nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with an affinity constant of 13.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-CD19 (FMC63), scFv, His Tag with an affinity constant of 74.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-BCMA scFv,His Tag with an affinity constant of 6.56 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-Human BCMA MAb (human IgG1) with an affinity constant of 14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
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