Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag

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Cat. No. / Size
Price
Qty
OV6-V81G9-25ug
$300.00
OV6-V81G9-200ug
$1110.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    OV16 (Onchocerca volvulus)

  • Source

    Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag (OV6-V81G9) is expressed from E. coli cells. It contains AA Lys 17 - Asp 197 (Accession # P31729-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    OV16 (Onchocerca volvulus) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a GST tag.

    The protein has a calculated MW of 48.8 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE).

    The protein is designed as a dimer.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    OV16 (Onchocerca volvulus) SDS-PAGE

    Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    OV16 (Onchocerca volvulus) SEC-MALS

    The purity of Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag (Cat. No. OV6-V81G9) is more than 90% and the molecular weight of this protein is around 90-105 kDa verified by SEC-MALS.

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Background

The OV16 antigen, localizes to the hypodermis, cuticle, and uterus of female O. volvulus is regarded as a highly sensitive and specific antigen for detection of IgG4 in people with prior exposure to onchocerciasis. It has an open reading frame that encodes a 152- amino acid polypeptide containing a 16-amino acid putative signal peptide. Enzyme-linked Immunosorbent Assay serological testing of OV-16 antigen is a diagnostic tool for determining effective elimination of the parasite.

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