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Your Position: Home > All Other Proteins > OV16 > OV6-V81G9

Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag

  • Synonym
    OV16,OV-16 antigen
  • Source
    Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag (OV6-V81G9) is expressed from E.coli cells. It contains AA Lys 17 - Asp 197 (Accession # P31729-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Lys 17 - Asp 197) P31729-1

    This protein carries an Avi tag at the N-terminus, followed by a GST tag..

    The protein has a calculated MW of 48.6 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag (Cat. No. OV6-V81G9) SDS-PAGE gel

Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag (Cat. No. ) MALS images

The purity of Biotinylated Onchocerca volvulus OV16 protein, Avi,GST Tag (Cat. No.OV6-V81G9) was more than 90% and the molecular weight of this protein is around 90-105 kDa verified by SEC-MALS.

  • Background
    The OV16 antigen, localizes to the hypodermis, cuticle, and uterus of female O. volvulus is regarded as a highly sensitive and specific antigen for detection of IgG4 in people with prior exposure to onchocerciasis. It has an open reading frame that encodes a 152- amino acid polypeptide containing a 16-amino acid putative signal peptide. Enzyme-linked Immunosorbent Assay serological testing of OV-16 antigen is a diagnostic tool for determining effective elimination of the parasite.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Price(USD) : $290.00

    Price(USD) : $980.00

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