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Your Position: Home > Binding antibody > Nucleocapsid protein > NUN-S47L8

Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47)

Recommended Hot Products: SP-11 Streptavidin Coated Plates, Clear, 96-Well  STN-N5116 Recombinant Streptavidin  SMB-B01 Magnetic Beads™ Streptavidin 
Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Source
    Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (NUN-S47L8) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
  • Isotype
    Mouse IgG1 / kappa
  • Specificity
    The cross-reactivity with other coronaviruses has not been tested yet.
  • Application
    This antibody can be paired with other Anti-SARS-CoV-2 Nucleocapsid antibodies to detect SARS-CoV-2 Nucleocapsid protein in sandwich ELISA or LFA assay.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-S47L8) SDS-PAGE gel

Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-S47L8) ELISA bioactivity

Detection SARS-CoV-2 Nucleocapsid Protein by Sandwich ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody (Cat. No. NUN-S46) at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein (Cat. No. NUN-C52). And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody (Cat. No. NUN-S47L8) at 0.05 µg/mL. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL (QC tested).

  • Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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