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Your Position: Home > All Other Proteins > NKG2A & CD94 > NC4-H82F5

Biotinylated Human NKG2A&CD94 Protein, Fc,Avitag™

  • Synonym
    NKG2A & CD94
  • Source
    Biotinylated Human NKG2A&CD94, Fc,Avitag™ (NC4-H82F5) is expressed from human 293 cells (HEK293). It contains AA Ala 113 - Leu 233 | Asp 57-Ile 179 (Accession # P26715-1(NKG2A) & Q13241-1(CD94)).
    Predicted N-terminus: Ala 113 | Asp 57
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 40.4 kDa | 40.4 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human NKG2A&CD94, Fc,Avitag™ (Cat. No. NC4-H82F5) SDS-PAGE gel

Biotinylated Human NKG2A&CD94, Fc,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human NKG2A&CD94, Fc,Avitag™Biotinylated Human NKG2A&CD94, Fc,Avitag™ (Cat. No. NC4-H82F5) ELISA bioactivity

Immobilized Biotinylated Human NKG2A&CD94, Fc,Avitag (Cat. No. NC4-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate. can bind various dilution ratio of HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE (QC tested).

  • Background
    CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. NKG2A/CD159a is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors that inhibits innate immune system activation. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA)-E on target cells.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $1380.00

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    Datasheet & Documentation

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    DMF (Drug Master File)

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