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Your Position: Home > All Other Proteins > KREMEN1 > KR1-H5253

Human Kremen-1 protein, Fc Tag

  • Synonym
    KREMEN, KRM1,Dickkopf receptor/Kringle domain-containing transmembrane protein 1/Kringle-containing protein marking the eye and the nose
  • Source
    Human Kremen-1, Fc Tag (KR1-H5253) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Thr 392 (Accession # Q96MU8-1).
    Predicted N-terminus: Arg 21
  • Molecular Characterization
    Online(Arg 21 - Thr 392) Q96MU8-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 67.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Kremen-1, Fc Tag (Cat. No. KR1-H5253) SDS-PAGE gel

Human Kremen-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Kremen-1, Fc TagHuman Kremen-1, Fc Tag (Cat. No. KR1-H5253) ELISA bioactivity

Immobilized Human Dkk-1, His Tag (Cat. No. DK1-H5221) at 2 μg/mL (100 μL/well) can bind Human Kremen-1, Fc Tag (Cat. No. KR1-H5253) with a linear range of 0.02-0.313 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human KREMEN1, Fc Tag (Cat. No. KR1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 283 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human KREMEN1, Fc Tag (Cat. No. KR1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein with an affinity constant of 114 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human KREMEN1, Fc Tag (Cat. No. KR1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein NTD, His Tag (Cat. No. S1D-C52H6) with an affinity constant of 241 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Kremens are high-affinity receptors for Dickkopf 1 (Dkk1) and regulate the Wnt/β-catenin signaling pathway by down-regulating the low-density lipoprotein receptor-related protein 6 (LRP6). Kremen1 is considered as one component of a membrane complex modulating canonical Wnt signalling through LRP6 in vertebrates. Additionally, KREMEN1 (KRM1) has also been identified as a functional receptor for Coxsackievirus A10 (CV-A10), a causative agent of hand-foot-and-mouth disease (HFMD).
  • References
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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