Human Kremen-1 protein, Fc Tag

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KR1-H5253-100ug
$450.00
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KR1-H5253-1mg (250ug X 4)
$3,170.00
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KR1-H5253
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Synonyms

KREMEN, KRM1,Dickkopf receptor, Kringle domain-containing transmembrane protein 1, Kringle-containing protein marking the eye and the nose

Source

Human Kremen-1, Fc Tag (KR1-H5253) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Thr 392 (Accession # Q96MU8-1).

Predicted N-terminus: Arg 21

Molecular Characterization

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 67.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Kremens are high-affinity receptors for Dickkopf 1 (Dkk1) and regulate the Wnt/β-catenin signaling pathway by down-regulating the low-density lipoprotein receptor-related protein 6 (LRP6). Kremen1 is considered as one component of a membrane complex modulating canonical Wnt signalling through LRP6 in vertebrates. Additionally, KREMEN1 (KRM1) has also been identified as a functional receptor for Coxsackievirus A10 (CV-A10), a causative agent of hand-foot-and-mouth disease (HFMD).