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Your Position: Home > Viral Proteins > S2 protein > S2N-C52E8

Biotinylated SARS-CoV-2 (COVID-19) S2 protein, His,Avitag™

  • Synonym
    Spike,S2 protein,Spike glycoprotein Subunit2,S glycoprotein Subunit2,Spike protein S2
  • Source
    Biotinylated SARS-CoV-2 S2 protein, His,Avitag™ (S2N-C52E8) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1).
    Predicted N-terminus: Ser 686
  • Molecular Characterization
    Online(Ser 686 - Pro 1213) QHD43416.1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 61.5 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated SARS-CoV-2 S2 protein, His,Avitag™ (Cat. No. S2N-C52E8) SDS-PAGE gel

Biotinylated SARS-CoV-2 S2 protein, His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated SARS-CoV-2 S2 protein, His,Avitag™Biotinylated SARS-CoV-2 S2 protein, His,Avitag™ (Cat. No. S2N-C52E8) ELISA bioactivity

Immobilized Biotinylated SARS-CoV-2 S2 protein, His,Avitag (Cat. No. S2N-C52E8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate. can bind SARS-CoV-2 Spike S2 Antibody, Human IgG1 with a linear range of 0.4-6 ng/mL (QC tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • References
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

  • Purchaser Notification
    Important Licensing Information: This product may be covered by Limited Use Label License(s). By use of this product, you accept the terms and conditions of all applicable Limited Use Label License(s).
  • Limited Use Label License No. 18: Engineered Coronavirus Spike Proteins
    This product and its use is the subject of U.S. Provisional Patent Application No. 63/032,502 and sold under a license from The University of Texas at Austin. Rights to use this product are limited to research and clinical diagnostics, and expressly exclude the right to use in human therapeutics, vaccines, and any use involving the administration of this product to humans.

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Price(USD) : $350.00

Price(USD) : $1380.00

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