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Your Position: Home > Immune Checkpoint Proteins > Nectin-4 > NE4-HF2H3

FITC-Labeled Human Nectin-4 Protein, His Tag

  • Synonym
    NECTIN4,LNIR, PRR4, PVRL4
  • Source
    FITC-Labeled Human Nectin-4, His Tag (NE4-HF2H3) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1). It is the FITC labeled form of Human Nectin-4, His Tag (NE4-H52H3).
    Predicted N-terminus: Gly 32
  • Molecular Characterization
    Online(Gly 32 - Ser 349) Q96NY8-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 36.0 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1-3.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human Nectin-4, His Tag (Cat. No. NE4-HF2H3) SDS-PAGE gel

FITC-Labeled Human Nectin-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
FITC-Labeled Human Nectin-4, His TagFITC-Labeled Human Nectin-4, His Tag (Cat. No. NE4-HF2H3) ELISA bioactivity

Immobilized Anti-Nectin-4 Antibody, Mouse IgG1 at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human Nectin-4, His Tag (Cat. No. NE4-HF2H3) with a linear range of 10-78 ng/mL (QC tested).

  • Background
    Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $319.00

Price(USD) : $1980.00

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  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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