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Your Position: Home > All Other Proteins > CD3E & CD3G > CDG-M52D2

Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (MALS verified)

  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (CDG-M52D2) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # Q22646-1(CD3E) & Q11942-1(CD3G)).
    Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
  • Molecular Characterization
    Online(Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G)) Q22646-1 (CD3E) & Q11942-1 (CD3G)

    Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 15.1 kDa (CD3E) and 15.2 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-22 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDG-M52D2) SDS-PAGE gel

Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (Cat. No. ) MALS images

The purity of Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag(Cat. No. CDG-M52D2) was more than 85% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Bioactivity-ELISA
Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag TagMouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDG-M52D2) ELISA bioactivity

Immobilized Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDG-M52D2) at 1 μg/mL (100 μL/well) can bind CD3e Monoclonal Antibody (145-2C11) with a linear range of 0.1-4 ng/mL (QC tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $2310.00

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