Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
SPD-C82E8-200ug
$1615.00
SPD-C82E8-1mg
$6665.00
SPD-C82E8-10mg (5mg X 2)
$48450.00
SPD-C82E8-100mg
$363385.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

  • Source

    Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (SPD-C82E8) is expressed from human 293 cells (HEK293).

    Predicted N-terminus: Arg 319

    Request for sequence

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM PB, pH7.4.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Spike RBD SDS-PAGE

    Biotinylated SARS-CoV-2 S protein RBD, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Spike RBD SEC-MALS

    The purity of Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E8) is more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Spike RBD ELISA

    Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E8) with a linear range of 10-313 ng/mL (QC tested).

    Protocol

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Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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