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Your Position: Home > Membrane Protein > CD133 > CD3-H52H1

Human CD133 Full Length Protein, His Tag (Nanodisc)

  • Synonym
    CD133,PROM1,PROML1,Prominin-1,AC133
  • Source
    Human CD133 Full Length, His Tag (Nanodisc)(CD3-H52H1) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - His 865 (Accession # O43490-1 ).
    Predicted N-terminus: Gly 20
  • Molecular Characterization
    CD133 Structure

    The CD133 carries a polyhistidine tag at the C-terminus with calculated MW of 97.2 kDa and migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H81Q5) is sold separately and not included in protein, you can follow this link for product information.
SDS-PAGE
CD133 SDS-PAGE

Human CD133 Full Length, His Tag (Nanodisc) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 CD133 ELISA

Immobilized Human CD133 Full Length, His Tag (Nanodisc) (Cat. No. CD3-H52H1) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD133 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

Bioactivity-FACS
 CD133 FACS

2e5 of Anti-CD133 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human CD133 Full Length, His Tag (Nanodisc) (Cat. No. CD3-H52H1) and negative control protein respectively, washed and then followed by PE-anti-His Tag antibody and analyzed with FACS (QC tested).

  • Background
    Prominin-1 is also known as CD133, Antigen AC133, PROM1, PROML1 and MSTP061. Is used as marker for hematopoietic stem and progenitor cells (HSPC) for somatic stem cell isolation. May play a role in cell differentiation, proliferation and apoptosis . Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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