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Product Details
Synonyms
TACSTD2, GA733-1, M1S1, TROP2
Source
Rhesus macaque TROP-2 Protein, His Tag (TR2-R52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274 (Accession # XP_001114599.1).
Predicted N-terminus: Gln 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.4 kDa. The protein migrates as 38-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rhesus macaque TROP-2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Rhesus macaque TROP-2 Protein, His Tag (Cat. No. TR2-R52H3) is more than 85% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Rhesus macaque TROP-2 Protein, His Tag (Cat. No. TR2-R52H3) at 1 μg/mL (100 μL/well) can bind Mouse Monoclonal Antibody Against Human TROP-2, Mouse IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Captured Trop2 antibody on CM5 chip via anti-mouse antibodies surface can bind Rhesus macaque TROP-2 Protein, His Tag (Cat. No. TR2-R52H3) with an affinity constant of 5.48 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor-associated calcium signal transducer 2
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
47 Details
- Highest Development Stage：
Approved
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