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Your Position: Home > Immune Checkpoint Proteins > Nectin-4 > NE4-M5257

Mouse Nectin-4 Protein, Fc Tag (MALS verified)

  • Synonym
    NECTIN4,LNIR, PRR4, PVRL4
  • Source
    Mouse Nectin-4, Fc Tag (NE4-M5257) is expressed from human 293 cells (HEK293). It contains AA Gly 31 - Ser 347 (Accession # Q8R007-1).
    Predicted N-terminus: Gly 31
  • Molecular Characterization
    Online(Gly 31 - Ser 347) Q8R007-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 60.8 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) SDS-PAGE gel

Mouse Nectin-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Mouse Nectin-4, Fc Tag (Cat. No. ) MALS images

The purity of Mouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) was more than 85% and the molecular weight of this protein is around 125-138 kDa verified by SEC-MALS.

Bioactivity-ELISA
Mouse Nectin-4, Fc TagMouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) ELISA bioactivity

Immobilized Human Nectin-1, His Tag (Cat. No. PV1-H5223) at 5 μg/mL (100 μL/well) on an Nickel Coated plate can bind Mouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) with a linear range of 0.156-2.5 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) on Protein A Biosensor, can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with an affinity constant of 278 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested)

  • Background
    Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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