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Your Position: Home > Protein > LGR5 > LG5-H52H3

Human LGR5 / GPR49 protein, His Tag

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  • Synonym
    LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5
  • Source
    Human LGR5, His Tag(LG5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Arg 561 (Accession # O75473-1 ).
  • Molecular Characterization
    LGR5 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 62.0 kDa. The protein migrates as 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LGR5 SDS-PAGE

Human LGR5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 LGR5 ELISA

Immobilized Human R-Spondin 3 Protein, Fc Tag (Cat. No. RS3-H5253) at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with a linear range of 10-78 ng/mL (QC tested).

 LGR5 ELISA

Immobilized Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Bioactivity-SPR
 LGR5 SPR

Human R-Spondin 3 (22-146), His Tag (Cat. No. RS6-H5222) with immobilized on CM5 Chip can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with an affinity constant of 18.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
  • Clinical and Translational Updates

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ETD of in-stock products: 2 business days

Price(USD) : $368.00

Price(USD) : $2667.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 2 Clinical

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