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Your Position: Home > All Other Proteins > IL-6 R alpha > ILR-H4223

Human IL-6 R alpha / CD126 Protein, His Tag

  • Synonym
    IL6R,CD126,IL-6R-1,IL-6RA,IL6RA,IL-6R-alpha,IL6RQ,gp80
  • Source
    Human IL-6 R alpha, His Tag (ILR-H4223) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1).
    Predicted N-terminus: Leu 20
  • Molecular Characterization
    Online(Leu 20 - Pro 365) NP_000556.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 39.4 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) SDS-PAGE gel

Human IL-6 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA
Human IL-6 R alpha, His TagHuman IL-6 R alpha, His Tag (Cat. No. ILR-H4223) ELISA bioactivity

Immobilized ActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) at 5 μg/mL (100 μL/well) can bind Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) with a linear range of 1-16 ng/mL (QC tested).

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