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>IL-6 R alpha
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.4 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-6 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Loaded Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) on HIS1K Biosensor, can bind Human IL-6, premium grade(Cat. No. IL6-H4218) with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Easy and rapid binding assay for functional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system
Authors: Tanemura Y, et al.
Journal: Biology (Basel) 2015
Application: Pulldown assay
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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