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Your Position: Home > All Other Proteins > CD3E & CD3G > CDG-C8219

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)

  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (CDG-C8219) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G) (Accession # Q95LI5-1(CD3E) & Q95LI7-1(CD3G)).
    Predicted N-terminus: Gln 22 (CD3E) & Gln 23 (CD3G)
  • Molecular Characterization
    Online(Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G)) Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)

    Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 17.7 kDa (CD3E) and 15.4 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-20 kDa and 22-24 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-C8219) SDS-PAGE gel

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. ) MALS images

The purity of Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-C8219) was more than 85% and the molecular weight of this protein is around 38-53 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag TagBiotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-C8219) ELISA bioactivity

Immobilized Bispecific T cell Engager (CD3 X BCMA) (Cat. No. CDA-H52Hb) at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No.CDG-C8219) with a linear range of 0.98-15.6 ng/mL (QC tested).

Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag TagBiotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-C8219) ELISA bioactivity

Immobilized Bispecific T cell Engager (CD3 X BCMA) at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No.CDG-C8219) with a linear range of 0.98-15.6 ng/mL (Routinely tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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