0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > Neuropilin-1 > NR1-H5253

Human Neuropilin-1 / NRP1 / CD304 Protein, Mouse IgG2a Fc Tag (MALS verified)

  • Synonym
    NRP1,Neuropilin-1,NRP,VEGF165R,CD304
  • Source
    Human Neuropilin-1, Mouse IgG2a Fc Tag (NR1-H5253) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Lys 644 (Accession # AAH07533.1).
    Predicted N-terminus: Phe 22
  • Molecular Characterization
    Online(Phe 22 - Lys 644) AAH07533.1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 96.7 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Neuropilin-1, Mouse IgG2a Fc Tag (Cat. No. NR1-H5253) SDS-PAGE gel

Human Neuropilin-1, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Neuropilin-1, Mouse IgG2a Fc Tag (Cat. No. ) MALS images

The purity of Human Neuropilin-1, Mouse IgG2a Fc Tag(Cat. No. NR1-H5253) was more than 90% and the molecular weight of this protein is around 230-250 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Neuropilin-1, Mouse IgG2a Fc TagHuman Neuropilin-1, Mouse IgG2a Fc Tag (Cat. No. NR1-H5253) ELISA bioactivity

Immobilized Human VEGF165, His Tag (Cat. No. VE5-H5248) at 2 μg/mL (100 μL/well) can bind Human Neuropilin-1, Mouse IgG2a Fc Tag (Cat. No. NR1-H5253) with a linear range of 0.2-5 ng/mL (QC tested).

  • Background
    Neuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 2 Clinical

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop