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Product Details
Synonyms
CD3E & CD3D, CD3 delta & CD3 epsilon
Source
Rabbit CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc (CDD-R5259) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Thr 120 (CD3E) & Thr 22 - Ser 107 (CD3D) (Accession # Q9TUF9-1 (CD3E) & A0A5F9D3I4-1 (CD3D)).
Predicted N-terminus: Gln 22 (CD3E) & Thr 22 (CD3D)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Rabbit CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.4 kDa (CD3E) and 40.8 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The reducing (R) protein migrates as 50 kDa and 60 kDa due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rabbit CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rabbit CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc (Cat. No. CDD-R5259) is more than 85% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.
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Customer Reviews Writing Reviews
S***r2022-06-10
LikesI had ordered this along with a number of other Acro products, based on good previous experiences with quality and delivery speed, for an immunization campaign and downstream testing. For us the purity of the product and low endotoxin content were key deciders when choosing to go which product to go for – so it was great to see this provided by Acro. We had a some delay in receiving part of the order, due to amount ordered, but technical support were great and we were really happy when we received the product and were able to use it, thanks very much Acro!
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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