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Your Position: Home > Viral Proteins > Spike RBD > SPD-C52Hn

SARS-CoV-2 (COVID-19) S protein RBD (N501Y), His Tag (MALS verified)

  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
  • Source
    SARS-CoV-2 S protein RBD (N501Y), His Tag (SPD-C52Hn) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(N501Y)). N501Y mutaion was identified in both the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom and SARS-CoV-2 variant (known as 20C/501Y.V2 or B.1.351 lineage) which emerged in South Africa.
    Predicted N-terminus: Arg 319
  • Molecular Characterization
    Online(Arg 319 - Lys 537) QHD43416.1 (N501Y)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) SDS-PAGE gel

SARS-CoV-2 S protein RBD (N501Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. ) MALS images

The purity of SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) was more than 90% and the molecular weight of this protein is around 28-38 kDa verified by SEC-MALS.

Bioactivity-ELISA
SARS-CoV-2 S protein RBD (N501Y), His TagSARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) with a linear range of 2-39 ng/mL (QC tested).

SARS-CoV-2 S protein RBD (N501Y), His TagSARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) ELISA bioactivity

Immobilized SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M180)) with a linear range of 0.1-3 ng/mL (Routinely tested).

SARS-CoV-2 S protein RBD (N501Y), His TagSARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) ELISA bioactivity

Serial dilutions of Monoclonal Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M180) were added into SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52H) : Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) binding reactions. The half maximal inhibitory concentration (IC50) is 1.13169 (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) with an affinity constant of 1.24 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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