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Your Position: Home > Receptor Tyrosine Kinases > Her2 > HE2-H52H9

Human Her2 / ErbB2 (23-510) Protein, His Tag (MALS verified)

  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    Human Her2 (23-510), His Tag (HE2-H52H9) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Ala 510 (Accession # P04626-1).
    Predicted N-terminus: Thr 23
  • Molecular Characterization
    Online(Thr 23 - Ala 510 ) P04626-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.6 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Her2 (23-510), His Tag (Cat. No. HE2-H52H9) SDS-PAGE gel

Human Her2 (23-510), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Her2 (23-510), His Tag (Cat. No. ) MALS images

The purity of Human Her2 (23-510), His Tag(Cat. No. HE2-H52H9) was more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Her2 (23-510), His TagHuman Her2 (23-510), His Tag (Cat. No. HE2-H52H9) ELISA bioactivity

Immobilized Human Her2 (23-510), His Tag (Cat. No. HE2-H52H9) at 1 μg/mL (100 μL/well) can bind Pertuzumab Biosimilar with a linear range of 0.1-4 ng/mL (QC tested).

Human Her2 (23-510), His TagHuman Her2 (23-510), His Tag (Cat. No. HE2-H52H9) ELISA bioactivity

Immobilized Human Her2 (23-510), His Tag (Cat. No. HE2-H52H9) at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (Her2 × Her3) with a linear range of 0.2-3 ng/mL (Routinely tested).

  • Background
    Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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