Biotinylated Human PSCA Protein, His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
PSA-H82E3-25ug
$415.00
PSA-H82E3-200ug
$1880.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    PSCA, UNQ206, PRO232

  • Source

    Biotinylated Human PSCA, His,Avitag (PSA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 12 - Ser 86 (Accession # O43653-1).

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  • Molecular Characterization

    PSCA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 12.1 kDa. The protein migrates as 20-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    PSCA SDS-PAGE

    Biotinylated Human PSCA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    PSCA SEC-MALS

    The purity of Biotinylated Human PSCA, His,Avitag (Cat. No. PSA-H82E3) is more than 95% and the molecular weight of this protein is around 18-25 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     PSCA ELISA

    Immobilized Biotinylated Human PSCA, His,Avitag (Cat. No. PSA-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-PSCA Antibody,Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  • Bioactivity-FACS

     PSCA FACS

    2e5 of anti-PSCA CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human PSCA, His,Avitag (Cat. No. PSA-H82E3) and negative control protein respectively, washed and then followed by PE-SA antibody and analyzed with FACS (Routinely tested).

    Protocol

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Background

The Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol (GPI)-anchored protein, plays an important role in tumorigenesis. The prostate stem cell antigen (PSCA) gene, which encodes a prostate-specific antigen (PSA), was identified as a gene involved in cell adhesion and proliferation. PSCA may be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. May act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits nicotine-induced signaling probably implicating alpha-3:beta-2- or alpha-7-containing nAChRs.

Recent Advances

 
Drug Development Progress
  • English Name:

    Prostate stem cell antigen

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    4 Details

  • Highest Development Stage:

    Phase 1 Clinical

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