SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag (MALS verified)

Spike, S protein, Spike glycoprotein, S glycoprotein

SARS-CoV-2 S protein, His Tag (SPN-C52H6) is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H mutations, which have become increasingly common in SARS-CoV-2 viruses from around the world. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. The HV69-70del / Y144del / N501Y / A570D / D614G / P681H / T716I / S982A / D1118H mutations were identified in the SARS-CoV-2 Alpha variant (Pango lineage: B.1.1.7; other names: 20I/501Y.V1 or VOC 202012/01).

Less than 1.0 EU per μg by the LAL method / rFC method.

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

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Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 12 months in lyophilized state;
• -70°C for 3 months under sterile conditions after reconstitution.