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Your Position: Home > Viral Proteins > S protein > SPN-C52H6

SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag (MALS verified)

  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein,COVID-19
  • Source
    SARS-CoV-2 S protein, His Tag (SPN-C52H6) is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H mutations, which have become increasingly common in SARS-CoV-2 viruses from around the world. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. HV69-70del / Y144del / N501Y / A570D / D614G / P681H / T716I / S982A / D1118H mutations were identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom.
    Predicted N-terminus: Val 16
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 137.8 kDa. The protein migrates as 150-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 S protein, His Tag (Cat. No. SPN-C52H6) SDS-PAGE gel

SARS-CoV-2 S protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
SARS-CoV-2 S protein, His Tag (Cat. No. ) MALS images

The purity of SARS-CoV-2 S protein, His Tag(Cat. No. SPN-C52H6) was more than 90% and the molecular weight of this protein is around 520-560 kDa kDa verified by SEC-MALS.

Bioactivity-ELISA
SARS-CoV-2 S protein, His TagSARS-CoV-2 S protein, His Tag (Cat. No. SPN-C52H6) ELISA bioactivity

Immobilized SARS-CoV-2 S protein, His Tag (Cat. No. SPN-C52H6) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-3 ng/mL (QC tested).

SARS-CoV-2 S protein, His TagSARS-CoV-2 S protein, His Tag (Cat. No. SPN-C52H6) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag (Cat. No. SPN-C52H6) with a linear range of 0.3-5 ng/mL (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • References
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

  • Purchaser Notification
    Important Licensing Information: This product may be covered by Limited Use Label License(s). By use of this product, you accept the terms and conditions of all applicable Limited Use Label License(s).
  • Limited Use Label License No. 18: Prefusion Coronavirus Spike Proteins
    This product and its use is the subject of one U.S. Provisional Patent Application No. 63/032,502. This product is sold under licenses from The University of Texas at Austin. Rights to use this product are limited to research only (including Non-clinical Diagnostic Kits), and expressly exclude the right to use in human therapeutics, vaccines and clinical diagnostics and any use involving the administration of this product to humans. The purchase of this product conveys to the buyer the non-transferrable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for other purposes shall be direct to The University of Texas at Austin, MCC Building, Mail Code R3500, 3925 West Braker Lane, Suite 1.9A, Austin, Texas 78759.

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