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Your Position: Home > ADC > Coagulation Factor III > TF3-H5253

Human Coagulation Factor III / Tissue Factor Protein, Fc Tag (active enzyme)

  • Synonym
    Coagulation Factor III,Tissue Factor,TF,F3,CD142
  • Source
    Human Tissue Factor, Fc Tag (TF3-H5253) is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Glu 251 (Accession # P13726-1).
    Predicted N-terminus: Ser 33
  • Molecular Characterization
    Online(Ser 33 - Glu 251) P13726-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 51.2 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Tissue Factor, Fc Tag (Cat. No. TF3-H5253) SDS-PAGE gel

Human Tissue Factor, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Tissue Factor, Fc TagHuman Tissue Factor, Fc Tag (Cat. No. TF3-H5253) ELISA bioactivity

Immobilized Human Tissue Factor, Fc Tag (Cat. No. TF3-H5253) at 1 μg/mL (100 μL/well) can bind Anti-TF Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).

Bioactivity

Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <5.5 μg/mL, as measured under the described conditions (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-TF mAb immobilized on CM5 Chip can bind Human Tissue Factor, Fc Tag (Cat. No. TF3-H5253) with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200) (Routinely tested).

  • Background
    Full-length tissue factor (TF) is a transmembrane receptor and cofactor for factor (F)VII/FVIIa. In addition to full-length TF, an alternative spliced (as) form of TF can be generated that lacks the transmembrane domain and is released fromcells. In contrast to TF, asTF has low procoagulant activity because it lacks the transmembrane domain. Tissue factor is expressed by cells around blood vessels, such as adventitial fibroblasts, and body surfaces, such as epithelial cells, and plays a critical role in hemostasis. TF also contributes to various forms of thrombosis. Many cancers, particularly adenocarcinomas, express high levels of TF. A high level of tumor TF expression is associated with poor prognosis in many types of cancers, including breast, prostate, colorectal, and pancreatic cancer.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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