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Your Position: Home > CD&BIOMARKER > IgG Fc > IGG-R5203

Rabbit IgG Fc Protein, Tag Free (MALS verified)

  • Synonym
    IgG Fc
  • Source
    Rabbit IgG Fc, Tag Free (IGG-R5203) is expressed from human 293 cells (HEK293). It contains AA Cys 105 - Lys 323 (Accession # P01870-1).
    Predicted N-terminus: Cys 105
  • Molecular Characterization
    Online(Cys 105 - Lys 323) P01870-1

    This protein carries no "tag". The protein has a calculated MW of 24.8 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) SDS-PAGE gel

Rabbit IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Rabbit IgG Fc, Tag Free (Cat. No. ) MALS images

The purity of Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) is more than 90% and the molecular weight of this protein is around 52-66 kDa verified by SEC-MALS.

Bioactivity-ELISA
Rabbit IgG Fc, Tag FreeRabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) ELISA bioactivity

Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.3-5 ng/mL (QC tested).

Rabbit IgG Fc, Tag FreeRabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) ELISA bioactivity

Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

  • Background
    Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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