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Your Position: Home > All Other Proteins > CD3E & CD3G > CDG-H52W5

Human CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Tag Free (MALS verified)

  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (CDG-H52W5) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1(CD3E) & P09693-1(CD3G)).
    Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
  • Molecular Characterization
    Online(Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)) P07766-1 (CD3E) & P09693-1 (CD3G)

    Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.9 kDa (CD3E) and 14.6 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 19 kDa and 21-28 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) SDS-PAGE gel

Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. ) MALS images

The purity of Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free(Cat. No. CDG-H52W5) was more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag FreeHuman CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) ELISA bioactivity

Immobilized Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.1-3 ng/mL (QC tested).

Human CD3E&CD3G Heterodimer Protein, His Tag&Tag FreeHuman CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) ELISA bioactivity

Immobilized Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) at 5 μg/mL (100 μL/well) can bind Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) with a linear range of 0.039-0.156 μg/mL (Routinely tested).

Human CD3E&CD3G Heterodimer Protein, His Tag&Tag FreeHuman CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) ELISA bioactivity

Immobilized UCHT1 at 1 μg/mL (100 μL/well) can bind Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) with a linear range of 0.8-3 ng/mL (Routinely tested).

Human CD3E&CD3G Heterodimer Protein, His Tag&Tag FreeHuman CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) ELISA bioactivity

Immobilized Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.4-3 ng/mL (Routinely tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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